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SA JOURNAL OF DIABETES & VASCULAR DISEASE

RESEARCH ARTICLE

VOLUME 13 NUMBER 2 • DECEMBER 2016

61

Cardioprotective and anti-hypertensive effects of

Prosopis

glandulosa

in rat models of pre-diabetes

B HUISAMEN, C GEORGE, D DIETRICH, S GENADE

Correspondence to: B Huisamen

MRC DDP, Parow; Faculty of Health Sciences, University of Stellenbosch,

Tygerberg, South Africa

e-mail:

bh3@sun.ac.za

B Huisamen, C George, S Genade

Department of Biomedical Sciences, Division of Medical Physiology, Faculty

of Health Sciences, University of Stellenbosch, Tygerberg, South Africa

D Dietrich

Department of Medical Biosciences, University of Western Cape, Bellville,

South Africa

Previously published in

Cardiovasc J Afr

2013;

24

: 10–16

S Afr J Diabetes Vasc Dis

2016;

13

: 61–67

Abstract

Aim:

Obesity and type 2 diabetes present with two

debilitating complications, namely, hypertension and heart

disease. The dried and ground pods of

Prosopis glandulosa

(commonly known as the Honey mesquite tree) which is part

of the Fabaceae (or legume) family are currently marketed

in South Africa as a food supplement with blood glucose-

stabilising and anti-hypertensive properties. We previously

determined its hypoglycaemic effects, and in the current

study we determined the efficacy of

P glandulosa

as anti-

hypertensive agent and its myocardial protective ability.

Methods:

Male Wistar rats were rendered either pre-diabetic

(diet-induced obesity: DIO) or hypertensive (high-fat diet:

HFD). DIO animals were treated with

P glandulosa

(100 mg/

kg/day for the last eight weeks of a 16-week period) and

compared to age-matched controls. Hearts were perfused

ex vivo to determine infarct size. Biometric parameters were

determined at the time of sacrifice. Cardiac-specific insulin

receptor knock-out (CIRKO) mice were similarly treated with

P glandulosa

and infarct size was determined. HFD animals

were treated with

P glandulosa

from the onset of the diet

or from weeks 12–16, using captopril (50 mg/kg/day) as the

positive control. Blood pressure was monitored weekly.

Results:

DIO rats and CIRKO mice:

P glandulosa

ingestion

significantly reduced infarct size after ischaemia–reperfusion.

Proteins of the PI-3-kinase/PKB/Akt survival pathway were

affected in a manner supporting cardioprotection. HFD

model:

P glandulosa

treatment both prevented and corrected

the development of hypertension, which was also reflected

in alleviation of water retention.

Conclusion:

P glandulosa

was cardioprotective and infarct

sparing as well as anti-hypertensive without affecting the

body weight or the intra-peritoneal fat depots of the animals.

Changes in the PI-3-kinase/PKB/Akt pathway may be causal

to protection. Results indicated water retention, possibly

coupled to vasoconstriction in the HFD animals, while

ingestion of

P glandulosa

alleviated both. We concluded that

treatment of pre-diabetes, type 2 diabetes or hypertension

with

P glandulosa

poses possible beneficial health effects.

Keywords:

Prosopis glandulosa

, hypertension, cardioprotection,

PKB, insulin resistance

Obesity and type 2 diabetes present with two debilitating

complications, namely, hypertension and heart disease. The dried

and ground pods of

Prosopis glandulosa

(commonly known as the

Honey mesquite tree) which is part of the Fabaceae (or legume)

family are currently marketed as a food supplement with blood

glucose stabilising and anti-hypertensive properties in South Africa.

In the past, the pods of this tree were used as the primary foodstuff

for the residents of the south-western regions of the North

American deserts and these trees are still widely distributed across

a large portion of the south-western United States.

1

The pods are

composed of 80% carbohydrate, 13% protein, 25% fibre and 3%

fat, and grinding of the plant is thought to improve its use.

2

Obesity is currently classified as a pandemic and is recognized as

the leading cause in the development of the metabolic syndrome.

The metabolic syndrome is described as a cluster of pathophysiology

outlined by the National Cholesterol Education Program’s Adult

Treatment Panel III (NCEP: ATP III) and the European Group for

the Study of Insulin Resistance, to include insulin resistance or

glucose intolerance (pre-diabetes), type 2 diabetes, hypertension

and atherogenic dyslipidaemia.

3,4

In addition, all of these factors

can be considered independent risk factors for the development of

cardiovascular disease.

3

According to the World Health Organisation (WHO), non-

communicable diseases such as heart disease, stroke, cancer,

chronic respiratory diseases and diabetes are currently (updated

June 2011) the leading causes of mortality in the world.

5

This

invisible epidemic is an under-appreciated cause of poverty and

hinders economic development in many countries. The burden

is growing and the number of people, families and communities

afflicted is increasing.

The time-line for development of overt type 2 diabetes is

described as developing over many years. The cardiovascular

consequences of this so-called ‘ticking clock’ hypothesis, starting

from obesity and culminating in type 2 diabetes, is present from the

early pre-diabetic stages.

6

In view of the scarcity and cost of modern oral hypoglycaemic

agents, plant-based therapies for the treatment of diabetes are

gaining considerable prominence.

7

According to these authors

more than 400 plant species have been described as having

hypoglycaemic activity. However, not all of these substances have

been researched scientifically to validate their efficacy.

We have researched a product from one such plant species,

consisting solely of the dried and ground pods of the plant

P glandulosa

, for hypoglycaemic properties.

8

In addition, potent